Construction of Pepstatin A-Conjugated ultrasmall SPIONs for targeted positive MR imaging of epilepsy-overexpressed P-glycoprotein

doi:10.1016/j.biomaterials.2019.119581

SIC OpenIR

	Construction of Pepstatin A-Conjugated ultrasmall SPIONs for targeted positive MR imaging of epilepsy-overexpressed P-glycoprotein
	Du, CJ; Wang, JH; Liu, XP; Li, HM; Geng, DY; Yu, LD; Chen, Y; Zhang, J
	2020-02-01
Source Publication	BIOMATERIALS
ISSN	0142-9612
Subtype	Article
Abstract	Surgical resection of the epileptogenic region is typically regarded to be practical and efficient for complete elimination of intractable seizures, which cannot be simply controlled by anti-epileptic drugs alone. To achieve a precision removal of the epileptogenic region and even a surgical cure, molecular imaging of epilepsy markers is highly essential for non-invasive accurate detection of the epileptogenic region. In this work, a peptide-targeted nanoprobe, based on ultrasmall superparamagnetic iron oxide nanoparticles (USPIONs), PA-USPIONs, was elaborately constructed to enable highly selective delivery and sensitive T-1-weighted positive magnetic resonance (MR) imaging of the epileptogenic region. Especially, Pepstatin A (PA), a small peptide which can specifically target to P-glycoprotein (P-gp) overexpressed at the epileptogenic region in a kainic acid (KA)-induced mice model of seizures, was conjugated onto the surface of PEGylated USPIONs. It has been demonstrated that the as-constructed PA-USPIONs nanoprobes have favorable T-1 contrast enhancement and high r(1) relaxivity compared with the clinically used T-1-MR contrast agent (Gd-DTPA) by systematic in vitro and vivo assessments. Importantly, the toxicity evaluation, especially to brains, was assessed by the histological as well as hematological examinations, demonstrating that the fabricated PA-USPIONs nanoprobes are featured with excellent biocompatibility, guaranteeing the further potential clinical application. This first report on the development of USPIONs as T-1-weighted MR contrast agents for active targeting of the epileptogenic region holds the high potential for precise resection of the according lesion in order to achieve therapeutic, often curative purposes.
DOI	10.1016/j.biomaterials.2019.119581
WOS Keyword	IRON-OXIDE NANOPARTICLES ; MEDIATED MULTIDRUG-RESISTANCE ; IN-VITRO ; LOCALIZATION ; BRAIN ; TUMOR ; MONODISPERSE ; SEIZURE ; FOCUS ; PET
Language	英语
WOS Research Area	Engineering ; Materials Science
Publisher	ELSEVIER SCI LTD
Citation statistics
Document Type	期刊论文
Identifier	http://ir.sic.ac.cn/handle/331005/28346
Collection	中国科学院上海硅酸盐研究所
Recommended Citation GB/T 7714	Du, CJ,Wang, JH,Liu, XP,et al. Construction of Pepstatin A-Conjugated ultrasmall SPIONs for targeted positive MR imaging of epilepsy-overexpressed P-glycoprotein[J]. BIOMATERIALS,2020.
APA	Du, CJ.,Wang, JH.,Liu, XP.,Li, HM.,Geng, DY.,...&Zhang, J.(2020).Construction of Pepstatin A-Conjugated ultrasmall SPIONs for targeted positive MR imaging of epilepsy-overexpressed P-glycoprotein.BIOMATERIALS.
MLA	Du, CJ,et al."Construction of Pepstatin A-Conjugated ultrasmall SPIONs for targeted positive MR imaging of epilepsy-overexpressed P-glycoprotein".BIOMATERIALS (2020).

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