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A lithium-containing biomaterial promotes chondrogenic differentiation of induced pluripotent stem cells with reducing hypertrophy
Hu, YQ; Chen, L; Gao, Y; Cheng, PZ; Yang, L; Wu, CT; Jie, Q
2020-02-21
Source PublicationSTEM CELL RESEARCH & THERAPY
Issue1
SubtypeArticle
AbstractBackgroundInduced pluripotent stem cells (iPSCs) exhibit limitless pluripotent plasticity and proliferation capability to provide an abundant cell source for tissue regenerative medicine. Thus, inducing iPSCs toward a specific differentiation direction is an important scientific question. Traditionally, iPSCs have been induced to chondrocytes with the help of some small molecules within 21-36days. To speed up the differentiation of iPSCs, we supposed to utilize bioactive ceramics to assist chondrogenic-induction process.MethodsIn this study, we applied ionic products (3.125 similar to 12.5mg/mL) of the lithium-containing bioceramic (Li2Ca4Si4O13, L2C4S4) and individual Li+ (5.78 similar to 23.73mg/L) in the direct chondrogenic differentiation of human iPSCs.ResultsCompared to pure chondrogenic medium and extracts of tricalcium phosphate (TCP), the extracts of L2C4S4 at a certain concentration range (3.125 similar to 12.5mg/mL) significantly enhanced chondrogenic proteins Type II Collagen (COL II)/Aggrecan/ SRY-Box 9 (SOX9) synthesis and reduced hypertrophic protein type X collagen (COL X)/matrix metallopeptidase 13 (MMP13) production in iPSCs-derived chondrocytes within 14days, suggesting that these newly generated chondrocytes exhibited favorable chondrocytes characteristics and maintained a low-hypertrophy state. Further studies demonstrated that the individual Li+ ions at the concentration range of 5.78 similar to 23.73mg/L also accelerated the chondrogenic differentiation of iPSCs, indicating that Li+ ions played a pivotal role in chondrogenic differentiation process.ConclusionsThese findings indicated that lithium-containing bioceramic with bioactive specific ionic components may be used for a promising platform for inducing iPSCs toward chondrogenic differentiation and cartilage regeneration.
DOI10.1186/s13287-020-01606-w
WOS KeywordMESENCHYMAL STROMAL CELLS ; CARTILAGE DEGRADATION ; CHONDROCYTES ; TISSUE ; PROLIFERATION ; GENERATION ; PROTECTS ; ALGINATE ; SCAFFOLD ; FGFR3
Language英语
WOS Research AreaCell Biology ; Research & Experimental Medicine
PublisherBMC
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Document Type期刊论文
Identifierhttp://ir.sic.ac.cn/handle/331005/28287
Collection中国科学院上海硅酸盐研究所
Recommended Citation
GB/T 7714
Hu, YQ,Chen, L,Gao, Y,et al. A lithium-containing biomaterial promotes chondrogenic differentiation of induced pluripotent stem cells with reducing hypertrophy[J]. STEM CELL RESEARCH & THERAPY,2020(1).
APA Hu, YQ.,Chen, L.,Gao, Y.,Cheng, PZ.,Yang, L.,...&Jie, Q.(2020).A lithium-containing biomaterial promotes chondrogenic differentiation of induced pluripotent stem cells with reducing hypertrophy.STEM CELL RESEARCH & THERAPY(1).
MLA Hu, YQ,et al."A lithium-containing biomaterial promotes chondrogenic differentiation of induced pluripotent stem cells with reducing hypertrophy".STEM CELL RESEARCH & THERAPY .1(2020).
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