Near-Infrared Voltage Nanosensors Enable Real-Time Imaging of Neuronal Activities in Mice and Zebrafish

doi:10.1021/jacs.0c01025

SIC OpenIR

	Near-Infrared Voltage Nanosensors Enable Real-Time Imaging of Neuronal Activities in Mice and Zebrafish
	Liu, JN; Zhang, RW; Shang, CF; Zhang, Y; Feng, Y; Pan, LM; Xu, B; Hyeon, T; Bu, WB; Shi, JL; Du, JL
	2020-04-29
Source Publication	JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
ISSN	0002-7863
Issue	17 Pages:7858
Subtype	Article
Abstract	Optical voltage sensors with the ability to monitor neuronal activities are invaluable tools for studying information processing of the brain. However, the current genetically encoded voltage indicators usually require high-power visible light for excitation and are limited to genetically addressable model animals. Here, we report a near-infrared (NIR)-excited nongenetic voltage nanosensor that achieves stable recording of neuronal membrane potential in intact animals. The nanosensor is composed of a Forster resonance energy transfer (FRET) pair, the outer membrane-anchored upconversion nanoparticle (UCNP), and the membrane-embedded dipicrylamine (DPA). The negative charge of DPA allows membrane potential fluctuation to affect the distance between the DPA and UCNP, therefore changing the FRET efficiency. Consequently, the emission intensity of the nanosensor can report the membrane potential. Using the nanosensor, we monitor not only electrically evoked changes in the membrane potential of cultured cells but also sensory responses of neurons in intact zebrafish and brain state-modulated subthreshold activities of cortical neurons in intact mice.
DOI	10.1021/jacs.0c01025
WOS Keyword	LUMINESCENT GOLD NANOPARTICLES ; UPCONVERTING NANOPARTICLES ; ELECTRICAL-ACTIVITY ; BRAIN-STIMULATION ; MEMBRANE ; NANOCRYSTALS ; CALCIUM
Language	英语
WOS Research Area	Chemistry
Publisher	AMER CHEMICAL SOC
Citation statistics
Document Type	期刊论文
Identifier	http://ir.sic.ac.cn/handle/331005/28109
Collection	中国科学院上海硅酸盐研究所
Recommended Citation GB/T 7714	Liu, JN,Zhang, RW,Shang, CF,et al. Near-Infrared Voltage Nanosensors Enable Real-Time Imaging of Neuronal Activities in Mice and Zebrafish[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2020(17):7858.
APA	Liu, JN.,Zhang, RW.,Shang, CF.,Zhang, Y.,Feng, Y.,...&Du, JL.(2020).Near-Infrared Voltage Nanosensors Enable Real-Time Imaging of Neuronal Activities in Mice and Zebrafish.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY(17),7858.
MLA	Liu, JN,et al."Near-Infrared Voltage Nanosensors Enable Real-Time Imaging of Neuronal Activities in Mice and Zebrafish".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY .17(2020):7858.

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