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Near-Infrared Voltage Nanosensors Enable Real-Time Imaging of Neuronal Activities in Mice and Zebrafish
Liu, JN; Zhang, RW; Shang, CF; Zhang, Y; Feng, Y; Pan, LM; Xu, B; Hyeon, T; Bu, WB; Shi, JL; Du, JL
2020-04-29
Source PublicationJOURNAL OF THE AMERICAN CHEMICAL SOCIETY
ISSN0002-7863
Issue17Pages:7858
SubtypeArticle
AbstractOptical voltage sensors with the ability to monitor neuronal activities are invaluable tools for studying information processing of the brain. However, the current genetically encoded voltage indicators usually require high-power visible light for excitation and are limited to genetically addressable model animals. Here, we report a near-infrared (NIR)-excited nongenetic voltage nanosensor that achieves stable recording of neuronal membrane potential in intact animals. The nanosensor is composed of a Forster resonance energy transfer (FRET) pair, the outer membrane-anchored upconversion nanoparticle (UCNP), and the membrane-embedded dipicrylamine (DPA). The negative charge of DPA allows membrane potential fluctuation to affect the distance between the DPA and UCNP, therefore changing the FRET efficiency. Consequently, the emission intensity of the nanosensor can report the membrane potential. Using the nanosensor, we monitor not only electrically evoked changes in the membrane potential of cultured cells but also sensory responses of neurons in intact zebrafish and brain state-modulated subthreshold activities of cortical neurons in intact mice.
DOI10.1021/jacs.0c01025
WOS KeywordLUMINESCENT GOLD NANOPARTICLES ; UPCONVERTING NANOPARTICLES ; ELECTRICAL-ACTIVITY ; BRAIN-STIMULATION ; MEMBRANE ; NANOCRYSTALS ; CALCIUM
Language英语
WOS Research AreaChemistry
PublisherAMER CHEMICAL SOC
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Document Type期刊论文
Identifierhttp://ir.sic.ac.cn/handle/331005/28109
Collection中国科学院上海硅酸盐研究所
Recommended Citation
GB/T 7714
Liu, JN,Zhang, RW,Shang, CF,et al. Near-Infrared Voltage Nanosensors Enable Real-Time Imaging of Neuronal Activities in Mice and Zebrafish[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2020(17):7858.
APA Liu, JN.,Zhang, RW.,Shang, CF.,Zhang, Y.,Feng, Y.,...&Du, JL.(2020).Near-Infrared Voltage Nanosensors Enable Real-Time Imaging of Neuronal Activities in Mice and Zebrafish.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY(17),7858.
MLA Liu, JN,et al."Near-Infrared Voltage Nanosensors Enable Real-Time Imaging of Neuronal Activities in Mice and Zebrafish".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY .17(2020):7858.
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