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Biointerface anisotropy modulates migration of breast cancer cell
Dai, J; Wang, YQ; Gong, JK; Yao, Y
2020-06-01
Source PublicationCOLLOIDS AND SURFACES B-BIOINTERFACES
ISSN0927-7765
SubtypeArticle
AbstractMigration of cancer cell is a cyclic process, which involves dynamic interaction between extracellular biointerface and cellular responds. In tumors, collagen as extracellular matrix reorganizes biointerface from curl and isotropic fibers to straightened and anisotropic fibers during tumorigenesis, yet how cell migration respond to topography of biointerface is unknown. In this research, we introduced a facile fabrication method on nanofibers of varying topography, which was mimicking the alignment of extracellular nanofibers, to examine the change of cytoskeleton during cell migration. We took advantage of breast carcinoma cell line (MDA-MB-231) for time-lapse imaging analysis. We found that biointerface anisotropy modulated morphology of cell and mediated the pattern of migration. Morphologically, cells on anisotropic nanofiber showed extending spindle shape. The trajectories of migration templated the topographic pattern on biointerface. Besides, aligned nanofiber induced caterpillar-like model of migration through protrusion - retraction cycle, which was indicated by periodical variation of aspect ratio and velocity of cells. The biointerface anisotropy triggered vimentin filaments and microtubule networks preferentially oriented along the alignment of nanofibers. And the velocity of cell mobility by vimentin, beta-catenin or CDC42 knockdown was significantly enhanced on aligned nanofibers. Thus, we implied that biointerface anisotropy modulated migration of breast cancer cell and it associated with reorganization of cytoskeleton filaments.
DOI10.1016/j.colsurfb.2020.110973
WOS KeywordEPITHELIAL-MESENCHYMAL TRANSITION ; VIMENTIN INTERMEDIATE-FILAMENTS ; LEADING-EDGE ; ACTIN ; METASTASIS ; NANOFIBER ; PERSISTENCE ; ACTIVATION ; DYNAMICS ; INVASION
Language英语
WOS Research AreaBiophysics ; Chemistry ; Materials Science
PublisherELSEVIER
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Document Type期刊论文
Identifierhttp://ir.sic.ac.cn/handle/331005/28023
Collection中国科学院上海硅酸盐研究所
Recommended Citation
GB/T 7714
Dai, J,Wang, YQ,Gong, JK,et al. Biointerface anisotropy modulates migration of breast cancer cell[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2020.
APA Dai, J,Wang, YQ,Gong, JK,&Yao, Y.(2020).Biointerface anisotropy modulates migration of breast cancer cell.COLLOIDS AND SURFACES B-BIOINTERFACES.
MLA Dai, J,et al."Biointerface anisotropy modulates migration of breast cancer cell".COLLOIDS AND SURFACES B-BIOINTERFACES (2020).
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