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An injectable continuous stratified structurally and functionally biomimetic construct for enhancing osteochondral regeneration
Zhu, Yanlun; Kong, Lingzhi; Farhadi, Fatemeh; Xia, Wei; Chang, Jiang; He, Yaohua; Li, Haiyan
2019-02-01
Source PublicationBIOMATERIALS
ISSN0142-9612
Volume192Pages:149
SubtypeArticle
AbstractOsteochondral regeneration with the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue still remains a great challenge. In this study, a construct containing an injectable continuous stratified scaffold and multiple cell systems was designed for enhancing osteochondral regeneration. Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration. The continuous SA phase and the stratified structure enable the scaffold to mimic the natural osteochondral structure. In addition, the SA/BG + BMSCs hydrogel could enhance the osteoblast differentiation of BMSCs by up regulating their alkaline phosphatase and collagen I gene expressions, and the SA/AG + ACs/BMSCs hydrogel could promote the chondrocyte differentiation of BMSCs by upregulating their Acan and collagen II gene expressions, which indicated that this stratified scaffold could mimic the natural osteochondral function. Furthermore, after the stratified construct was injected into a rat osteochondral defect model, obvious neonatal articular cartilage tissues and subchondral bone tissues with regular surface and highly integration with normal tissues could be observed. This structural and functional biomimetic construct, together with its proper swelling ratio, could not only stimulate the hyaline cartilage and subchondral bone regeneration in an entire osteochondral unit but also promote the integration between the newly formed tissues and the host tissue.
KeywordOsteochondral Continuous stratified scaffolds Injectable hydrogels Co-cultures
DOI10.1016/j.biomaterials.2018.11.017
Language英语
WOS Research AreaEngineering ; Materials Science
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sic.ac.cn/handle/331005/27367
Collection中国科学院上海硅酸盐研究所
Recommended Citation
GB/T 7714
Zhu, Yanlun,Kong, Lingzhi,Farhadi, Fatemeh,et al. An injectable continuous stratified structurally and functionally biomimetic construct for enhancing osteochondral regeneration[J]. BIOMATERIALS,2019,192:149.
APA Zhu, Yanlun.,Kong, Lingzhi.,Farhadi, Fatemeh.,Xia, Wei.,Chang, Jiang.,...&Li, Haiyan.(2019).An injectable continuous stratified structurally and functionally biomimetic construct for enhancing osteochondral regeneration.BIOMATERIALS,192,149.
MLA Zhu, Yanlun,et al."An injectable continuous stratified structurally and functionally biomimetic construct for enhancing osteochondral regeneration".BIOMATERIALS 192(2019):149.
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