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Micro- and Nanohemispherical 3D Imprints Modulate the Osteogenic Differentiation and Mineralization Tendency of Bone Cells
Zhu, Yizhou; Liu, Xiangmei; Wu, Jun; Wong, Tak Man; Feng, Xiaobo; Yang, Cao; Wu, Shuilin; Zheng, Yufeng; Liu, Xuanyong; Cheung, Kenneth M. C.; Yeung, Kelvin W. K.
2019-10-02
Source PublicationACS APPLIED MATERIALS & INTERFACES
ISSN1944-8244
Volume11Issue:39Pages:35513
SubtypeArticle
AbstractSurface topography has been reported to play a key role in modulating cell behaviors, yet the mechanism through which it modulates these behaviors is not fully understood, especially in the case of three-dimensional (3D) topographies. In this study, a series of novel hemispherical 3D imprints ranging from the nanoscale to the microscale were prepared on titanium (Ti) surfaces using a customized interfacial lithography method. Mouse embryo osteoblast precursor cells (MC3T3-E1) were selected to investigate the solitary effect of specific hemispherical 3D imprints on cellular behaviors. The results indicated that varied hemispherical 3D, imprints can affect the formation of filopodia and the arrangement of the cytoskeleton in different ways. Specifically, they can alter the spreading morphologies of cells and lead to deformation of the nucleus, which eventually affects cell proliferation and osteogenic differentiation. Cells cultured on different hemispherical 3D imprints exhibited promoted proliferation and osteogenic differentiation to different degrees; for example, cells cultured on 90 and 500 nm hemispherical imprints formed abundant filopodia and exhibited the highest alkaline phosphatase activity and osteogenic gene expression, respectively. Four week tibia implantation also confirmed that 90 nm hemispherical imprints improved the osteogenic ability in vivo compared with an unpatterned Ti substrate. In addition to promoted proliferation, colonization of more cells on the surface of implants and induction of rapid osteogenic differentiation can occur. Our work provides a rational way to balance cell proliferation and differentiation, which can accelerate bone integration of an implant and host tissue.
KeywordTi implants hemispherical 3D imprints preosteoblasts osteointegration micro-/nanoscale surface morphology
DOI10.1021/acsami.9b05521
Language英语
WOS Research AreaScience & Technology - Other Topics ; Materials Science
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sic.ac.cn/handle/331005/26782
Collection中国科学院上海硅酸盐研究所
Recommended Citation
GB/T 7714
Zhu, Yizhou,Liu, Xiangmei,Wu, Jun,et al. Micro- and Nanohemispherical 3D Imprints Modulate the Osteogenic Differentiation and Mineralization Tendency of Bone Cells[J]. ACS APPLIED MATERIALS & INTERFACES,2019,11(39):35513.
APA Zhu, Yizhou.,Liu, Xiangmei.,Wu, Jun.,Wong, Tak Man.,Feng, Xiaobo.,...&Yeung, Kelvin W. K..(2019).Micro- and Nanohemispherical 3D Imprints Modulate the Osteogenic Differentiation and Mineralization Tendency of Bone Cells.ACS APPLIED MATERIALS & INTERFACES,11(39),35513.
MLA Zhu, Yizhou,et al."Micro- and Nanohemispherical 3D Imprints Modulate the Osteogenic Differentiation and Mineralization Tendency of Bone Cells".ACS APPLIED MATERIALS & INTERFACES 11.39(2019):35513.
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