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Ti4+-immobilized chitosan-coated magnetic graphene oxide for the highly selective enrichment of phosphopeptides
Zhao, Yiman1; Zhang, Lingyi1; Chu, Zhanying1; Xiong, Zhichao2; Zhang, Weibing1
2017
发表期刊Analytical Methods
ISSN17599660
卷号9期号:3页码:443-449
摘要For in-depth analysis of phosphorylated proteomics, the highly sensitive and selective capture of phosphopeptides from intricate biological samples is extremely significant. In this work, a late-model immobilized metal ion affinity chromatography (IMAC) material was fabricated and applied to enriching phosphopeptides from biological samples by abundant Ti4+ions, which were chelated to crosslinked chitosan (CS) loaded on the surface of magnetic graphene oxide (GO) (denoted as mag GO-CS-Ti4+). The high amount of crosslinked CS endowed the IMAC material with highly hydrophilic properties and abundant active sites for Ti4+ions on the surface. These characteristics ensure the great performance of mag GO-CS-Ti4+in the selective enrichment of phosphopeptides from the tryptic digest of β-casein with high selectivity (phosphopeptides to non-phosphopeptides at a molar ratio of 1 : 400), high sensitivity (0.5 fmol), large enrichment capacity (66.6 mg g-1), prominent phosphopeptide recovery (93.11%), and excellent reusability. What's more, mag GO-CS-Ti4+was applied to capturing phosphopeptides from real biological samples, human serum from both healthy people and patients and nonfat milk. The results successfully manifested that the mag GO-CS-Ti4+could be a great affinity material for the enrichment of low-abundant phosphopeptides from biological samples. © 2017 The Royal Society of Chemistry.
DOI10.1039/c6ay02905a
EI入藏号20170403276337
EI主题词Titanium oxides
EI分类号461.2 Biological Materials and Tissue Engineering - 461.9 Biology - 531.1 Metallurgy - 761 Nanotechnology - 801 Chemistry - 804 Chemical Products Generally
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被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sic.ac.cn/handle/331005/25935
专题中国科学院上海硅酸盐研究所
作者单位1.Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai; 200237, China;
2.State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai; 200050, China
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Zhao, Yiman,Zhang, Lingyi,Chu, Zhanying,et al. Ti4+-immobilized chitosan-coated magnetic graphene oxide for the highly selective enrichment of phosphopeptides[J]. Analytical Methods,2017,9(3):443-449.
APA Zhao, Yiman,Zhang, Lingyi,Chu, Zhanying,Xiong, Zhichao,&Zhang, Weibing.(2017).Ti4+-immobilized chitosan-coated magnetic graphene oxide for the highly selective enrichment of phosphopeptides.Analytical Methods,9(3),443-449.
MLA Zhao, Yiman,et al."Ti4+-immobilized chitosan-coated magnetic graphene oxide for the highly selective enrichment of phosphopeptides".Analytical Methods 9.3(2017):443-449.
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