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Nano-layered magnesium fluoride reservoirs on biomaterial surfaces strengthen polymorphonuclear leukocyte resistance to bacterial pathogens
Guo, Geyong1; Zhou, Huaijuan2; Wang, Qiaojie1; Wang, Jiaxing1; Tan, Jiaqi1; Li, Jinhua2,3; Jin, Ping2; Shen, Hao1
2017
Source PublicationNanoscale
ISSN20403364
Volume9Issue:2Pages:875-892
AbstractBiomaterial-related bacterial infections cause patient suffering, mortality and extended periods of hospitalization, imposing a substantial burden on medical systems. In this context, understanding of nanomaterials-bacteria-cells interactions is of both fundamental and clinical significance. Herein, nano-MgF2films were deposited on titanium substrate via magnetron sputtering. Using this platform, the antibacterial behavior and mechanism of the nano-MgF2films were investigated in vitro and in vivo. It was found that, for S. aureus (CA-MRSA, USA300) and S. epidermidis (RP62A), the nano-MgF2films possessed excellent anti-biofilm activity, but poor anti-planktonic bacteria activity in vitro. Nevertheless, both the traditional SD rat osteomyelitis model and the novel stably luminescent mouse infection model demonstrated that nano-MgF2films exerted superior anti-infection effect in vivo, which cannot be completely explained by the antibacterial activity of the nanomaterial itself. Further, using polymorphonuclear leukocytes (PMNs), the critical immune cells of innate immunity, a complementary investigation of MgF2-bacteria-PMNs co-culturing revealed that the nano-MgF2films improved the antibacterial effect of PMNs through enhancing their phagocytosis and stability. To our knowledge, this is the first time of exploring the antimicrobial mechanism of nano-MgF2from the perspective of innate immunity both in vitro and in vivo. Based on the research results, a plausible mechanism is put forward for the predominant antibacterial effect of nano-MgF2in vivo, which may originate from the indirect immune enhancement effect of nano-MgF2films. In summary, this study of surface antibacterial design using MgF2nanolayer is a meaningful attempt, which can promote the host innate immune response to bacterial pathogens. This may give us a new understanding towards the antibacterial behavior and mechanism of nano-MgF2films and pave the way towards their clinical applications. © The Royal Society of Chemistry 2017.
DOI10.1039/c6nr07729c
EI Accession Number20170203241272
EI KeywordsMagnesium compounds
EI Classification Number461.9.1 Immunology - 761 Nanotechnology
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Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sic.ac.cn/handle/331005/25818
Collection中国科学院上海硅酸盐研究所
Affiliation1.Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai; 200233, China;
2.State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai; 200050, China;
3.University of Chinese Academy of Sciences, Beijing; 100049, China
Recommended Citation
GB/T 7714
Guo, Geyong,Zhou, Huaijuan,Wang, Qiaojie,et al. Nano-layered magnesium fluoride reservoirs on biomaterial surfaces strengthen polymorphonuclear leukocyte resistance to bacterial pathogens[J]. Nanoscale,2017,9(2):875-892.
APA Guo, Geyong.,Zhou, Huaijuan.,Wang, Qiaojie.,Wang, Jiaxing.,Tan, Jiaqi.,...&Shen, Hao.(2017).Nano-layered magnesium fluoride reservoirs on biomaterial surfaces strengthen polymorphonuclear leukocyte resistance to bacterial pathogens.Nanoscale,9(2),875-892.
MLA Guo, Geyong,et al."Nano-layered magnesium fluoride reservoirs on biomaterial surfaces strengthen polymorphonuclear leukocyte resistance to bacterial pathogens".Nanoscale 9.2(2017):875-892.
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