SIC OpenIR
YAP-mediated mechanotransduction regulates osteogenic and adipogenic differentiation of BMSCs on hierarchical structure
Pan, Houhua1,2,3; Xie, Youtao1; Zhang, Zequan1; Li, Kai1; Hu, Dandan1; Zheng, Xuebin1; Fan, Qiming2; Tang, Tingting2
2017
Source PublicationColloids and Surfaces B: Biointerfaces
ISSN09277765
Volume152Pages:344-353
AbstractHierarchical structure mimicking the natural bone microenvironment has been considered as a promising platform to regulate cell functions. We have previously fabricated hierarchical macropore/nanowire structure and evidence has shown that it can better manipulate the cytoskeleton status and osteogenic performance of osteoblasts. However, how cues of hierarchical structure are translated and ultimately linked to BMSC lineage commitment have still remained elusive, which hinders the accurate knowledge and further development of the hierarchical structure. In this study, bone marrow-derived mesenchymal stem cells (BMSCs) fate on hierarchical structure was investigated as well as the detailed mechanisms. It was shown that well-developed cytoskeleton and focal adhesion were observed for BMSCs on hierarchical structure, which was accompanied by enhanced osteogenic and depressed adipogenic potential. Evidence of increased YAP activity and nuclear translocation were exhibited on hierarchical structure and YAP knockdown inhibited osteogenic differentiation and promoted adipogenic differentiation induced by hierarchical structure. Further remove of cytoskeleton tension inhibited YAP function, which confirmed the key role of YAP-mediated mechanotransduction in the BMSC differentiation. These results together provide information of the stem cell fate commitment on hierarchical structure and a promising approach to design advanced biomaterials by focusing on specific mechanotransduction process. © 2017 Elsevier B.V.
DOI10.1016/j.colsurfb.2017.01.039
EI Accession Number20170503307431
EI KeywordsStem cells
EI Classification Number461.2 Biological Materials and Tissue Engineering - 921.2 Calculus
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sic.ac.cn/handle/331005/25728
Collection中国科学院上海硅酸盐研究所
Affiliation1.Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai; 200050, China;
2.Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai; 200011, China;
3.University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing; 100049, China
Recommended Citation
GB/T 7714
Pan, Houhua,Xie, Youtao,Zhang, Zequan,et al. YAP-mediated mechanotransduction regulates osteogenic and adipogenic differentiation of BMSCs on hierarchical structure[J]. Colloids and Surfaces B: Biointerfaces,2017,152:344-353.
APA Pan, Houhua.,Xie, Youtao.,Zhang, Zequan.,Li, Kai.,Hu, Dandan.,...&Tang, Tingting.(2017).YAP-mediated mechanotransduction regulates osteogenic and adipogenic differentiation of BMSCs on hierarchical structure.Colloids and Surfaces B: Biointerfaces,152,344-353.
MLA Pan, Houhua,et al."YAP-mediated mechanotransduction regulates osteogenic and adipogenic differentiation of BMSCs on hierarchical structure".Colloids and Surfaces B: Biointerfaces 152(2017):344-353.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Pan, Houhua]'s Articles
[Xie, Youtao]'s Articles
[Zhang, Zequan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Pan, Houhua]'s Articles
[Xie, Youtao]'s Articles
[Zhang, Zequan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Pan, Houhua]'s Articles
[Xie, Youtao]'s Articles
[Zhang, Zequan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.