SIC OpenIR
YAP-mediated mechanotransduction regulates osteogenic and adipogenic differentiation of BMSCs on hierarchical structure
Pan, Houhua1,2,3; Xie, Youtao1; Zhang, Zequan1; Li, Kai1; Hu, Dandan1; Zheng, Xuebin1; Fan, Qiming2; Tang, Tingting2
2017
发表期刊Colloids and Surfaces B: Biointerfaces
ISSN09277765
卷号152页码:344-353
摘要Hierarchical structure mimicking the natural bone microenvironment has been considered as a promising platform to regulate cell functions. We have previously fabricated hierarchical macropore/nanowire structure and evidence has shown that it can better manipulate the cytoskeleton status and osteogenic performance of osteoblasts. However, how cues of hierarchical structure are translated and ultimately linked to BMSC lineage commitment have still remained elusive, which hinders the accurate knowledge and further development of the hierarchical structure. In this study, bone marrow-derived mesenchymal stem cells (BMSCs) fate on hierarchical structure was investigated as well as the detailed mechanisms. It was shown that well-developed cytoskeleton and focal adhesion were observed for BMSCs on hierarchical structure, which was accompanied by enhanced osteogenic and depressed adipogenic potential. Evidence of increased YAP activity and nuclear translocation were exhibited on hierarchical structure and YAP knockdown inhibited osteogenic differentiation and promoted adipogenic differentiation induced by hierarchical structure. Further remove of cytoskeleton tension inhibited YAP function, which confirmed the key role of YAP-mediated mechanotransduction in the BMSC differentiation. These results together provide information of the stem cell fate commitment on hierarchical structure and a promising approach to design advanced biomaterials by focusing on specific mechanotransduction process. © 2017 Elsevier B.V.
DOI10.1016/j.colsurfb.2017.01.039
EI入藏号20170503307431
EI主题词Stem cells
EI分类号461.2 Biological Materials and Tissue Engineering - 921.2 Calculus
引用统计
文献类型期刊论文
条目标识符http://ir.sic.ac.cn/handle/331005/25728
专题中国科学院上海硅酸盐研究所
作者单位1.Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai; 200050, China;
2.Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai; 200011, China;
3.University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing; 100049, China
推荐引用方式
GB/T 7714
Pan, Houhua,Xie, Youtao,Zhang, Zequan,et al. YAP-mediated mechanotransduction regulates osteogenic and adipogenic differentiation of BMSCs on hierarchical structure[J]. Colloids and Surfaces B: Biointerfaces,2017,152:344-353.
APA Pan, Houhua.,Xie, Youtao.,Zhang, Zequan.,Li, Kai.,Hu, Dandan.,...&Tang, Tingting.(2017).YAP-mediated mechanotransduction regulates osteogenic and adipogenic differentiation of BMSCs on hierarchical structure.Colloids and Surfaces B: Biointerfaces,152,344-353.
MLA Pan, Houhua,et al."YAP-mediated mechanotransduction regulates osteogenic and adipogenic differentiation of BMSCs on hierarchical structure".Colloids and Surfaces B: Biointerfaces 152(2017):344-353.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Pan, Houhua]的文章
[Xie, Youtao]的文章
[Zhang, Zequan]的文章
百度学术
百度学术中相似的文章
[Pan, Houhua]的文章
[Xie, Youtao]的文章
[Zhang, Zequan]的文章
必应学术
必应学术中相似的文章
[Pan, Houhua]的文章
[Xie, Youtao]的文章
[Zhang, Zequan]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。