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Electrospinning of PVA/sericin nanofiber and the effect on epithelial-mesenchymal transition of A549 cells
Yan, Shanshan1,3,4; Li, Xiuchun1; Dai, Jing1; Wang, Yiqun1; Wang, Binbin2,4,5; Lu, Yi1; Shi, Jianlin1,3; Huang, Pengyu2; Gong, Jinkang1; Yao, Yuan1
2017
Source PublicationMaterials Science and Engineering C
ISSN09284931
Volume79Pages:436-444
AbstractThis research aims to investigate the cell-nanomaterial interaction between epithelial-mesenchymal transition of A549 cell and electrospinning nanofibers composed of polyvinyl alcohol (PVA)/silk sericin (SS). The electrospinning of regenerated nanofiber was performed with water as a spinning solvent and glutaraldehyde as a chemical cross-linker. Solution concentration, applied voltage and spin distances as well as other parameters were optimized to generate fine nanofibers with smooth surface in good homogeneity. From the scanning electron microscopy (SEM) analysis, the nanofibers had an average diameter of 200 nm. Epithelial-mesenchymal transition (EMT) is a process by which epithelial cells lose their cell polarity to become mesenchymal stem cells. This transition is affected by multiple biochemical and physical factors in cell metabolism cascade. Herein, we investigate the biophysical effect on A549 EMT by culturing cells on nanofibrous mats with different topography and composition. The cell viability was evaluated by biochemical assay and its morphology was observed with SEM. The results demonstrate that cells appropriately attached to the surface of the nanofibrous mats with extended morphology by their filopodia. Gene expression analysis was conducted by real-time PCR using multiple markers for detecting EMT: N-cadherin (NCad), Vimentin (Vim), Fibronectin (Fib) and Matrix metallopeptidase (MMP9). An increasing expression pattern was observed on NCad, Vim, Fib, with respect to a negative control as cell cultured on polystyrene dish. This result indicates the 200 nm PVA/SS nanofibers may induce A549 cells to process epithelial–mesenchymal transition during the culturing. © 2017 Elsevier B.V.
DOI10.1016/j.msec.2017.05.048
EI Accession Number20172103685554
EI KeywordsElectrospinning
EI Classification Number461.2 Biological Materials and Tissue Engineering - 461.9 Biology - 761 Nanotechnology - 801.2 Biochemistry - 815.1.1 Organic Polymers - 819.3 Fiber Chemistry and Processing - 933 Solid State Physics
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Document Type期刊论文
Identifierhttp://ir.sic.ac.cn/handle/331005/25665
Collection中国科学院上海硅酸盐研究所
Affiliation1.School of Physical Science and Technology, ShanghaiTech University, 393 Middle Huaxia Road, Pudong, Shanghai; 201210, China;
2.School of Life Science and Technology, ShanghaiTech University, 393 Middle Huaxia Road, Pudong, Shanghai; 201210, China;
3.Shanghai Institute of Ceramics, Chinese Academy of Science, 1295 Dingxi Road, Changning, Shanghai; 200050, China;
4.University of Chinese Academy of Sciences, 19 Yuquan Road, Shijingshan, Beijing; 100049, China;
5.Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Xuhui, Shanghai; 200031, China
Recommended Citation
GB/T 7714
Yan, Shanshan,Li, Xiuchun,Dai, Jing,et al. Electrospinning of PVA/sericin nanofiber and the effect on epithelial-mesenchymal transition of A549 cells[J]. Materials Science and Engineering C,2017,79:436-444.
APA Yan, Shanshan.,Li, Xiuchun.,Dai, Jing.,Wang, Yiqun.,Wang, Binbin.,...&Yao, Yuan.(2017).Electrospinning of PVA/sericin nanofiber and the effect on epithelial-mesenchymal transition of A549 cells.Materials Science and Engineering C,79,436-444.
MLA Yan, Shanshan,et al."Electrospinning of PVA/sericin nanofiber and the effect on epithelial-mesenchymal transition of A549 cells".Materials Science and Engineering C 79(2017):436-444.
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