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Synthesis of magnetic zwitterionic–hydrophilic material for the selective enrichment of N-linked glycopeptides
Zhao, Yiman1; Chen, Yajing1; Xiong, Zhichao2; Sun, Xudong1; Zhang, Quanqing3; Gan, Yangyang1; Zhang, Lingyi1; Zhang, Weibing1
2017
Source PublicationJournal of Chromatography A
ISSN00219673
Volume1482Pages:23-31
AbstractIn consideration of the close connection between glycopeptides and human diseases, the efficient method to separate and enrich glycopeptides from complex biological samples is urgently required. In the work, we developed a magnetic zwitterionic-hydrophilic material for highly effective separation and analysis of glycopeptides from complex samples. The Fe3O4particles were covered with a thick layer of polymer by one-step reflux-precipitation polymerization (RPP), subsequently decorated by Au nanoparticles (Au NPs) through in situ reduction and finally modified with zwitterionic groups. The abundant zwitterionic sites facilitate the selective enrichment of glycopeptides. Besides, the prepared Fe3O4@PGMA@Au-L-cys showed high detection sensitivity (5 fmol IgG digest), approving enrichment capacity (75 mg g−1), satisfactory enrichment recovery (89.8%), and great performance in the analysis and profiling of low-abundance N-linked glycopeptides. Furthermore, the prepared material was employed in the enrichment of glycopeptides in intricate biological samples, and 774 unique N-glycosylation sites from 411 N-glycosylated proteins were reliably identified in three replicate analyses of a 75 μg protein sample extracted from mouse liver, suggesting wide application prospect in glycoproteomics. © 2016 Elsevier B.V.
DOI10.1016/j.chroma.2016.12.054
EI Accession Number20170203238376
EI KeywordsPeptides
EI Classification Number461.2 Biological Materials and Tissue Engineering - 461.9 Biology - 701.2 Magnetism: Basic Concepts and Phenomena - 761 Nanotechnology - 801 Chemistry - 802.3 Chemical Operations - 804 Chemical Products Generally - 933 Solid State Physics
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Document Type期刊论文
Identifierhttp://ir.sic.ac.cn/handle/331005/25653
Collection中国科学院上海硅酸盐研究所
Affiliation1.Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai; 200237, China;
2.State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai; 200050, China;
3.Fudan University, Shanghai; 200433, China
Recommended Citation
GB/T 7714
Zhao, Yiman,Chen, Yajing,Xiong, Zhichao,等. Synthesis of magnetic zwitterionic–hydrophilic material for the selective enrichment of N-linked glycopeptides[J]. Journal of Chromatography A,2017,1482:23-31.
APA Zhao, Yiman.,Chen, Yajing.,Xiong, Zhichao.,Sun, Xudong.,Zhang, Quanqing.,...&Zhang, Weibing.(2017).Synthesis of magnetic zwitterionic–hydrophilic material for the selective enrichment of N-linked glycopeptides.Journal of Chromatography A,1482,23-31.
MLA Zhao, Yiman,et al."Synthesis of magnetic zwitterionic–hydrophilic material for the selective enrichment of N-linked glycopeptides".Journal of Chromatography A 1482(2017):23-31.
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